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cytof helios instrument  (fluidigm)


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    Structured Review

    fluidigm cytof helios instrument
    Cytof Helios Instrument, supplied by fluidigm, used in various techniques. Bioz Stars score: 93/100, based on 3453 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/helios+cytof+instrument/Helios/pmc12510166-459-6-9
    Average 93 stars, based on 3453 article reviews
    cytof helios instrument - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Software:

    Article Title: Investigating immune profile by CyTOF in patients with eosinophilic esophagitis after treatment with orodispersible budesonide
    Article Snippet: Prior to sample analysis, the cells were resuspended in MilliQ H2O to 1 × 106 cells/ml and 0.1X EQ Four Element Calibration Beads (Fluidigm) were added. .. Analyses were performed using a Helios CyTOF instrument with CyTOF Software v7.0. (Fluidigm) and samples were gated using FlowJo 10.8.0 software (Tree Star Inc., Ashland, OR) ( ). ..

    Article Title: In vivo single-cell high-dimensional mass cytometry analysis to track the interactions between Klebsiella pneumoniae and myeloid cells.
    Article Snippet: Cells were resuspended in CAS with 1 mM EDTA to a final concentration of 1 × 106 cells/mL, flushed through a 35 μM strainer, and supplemented with 1/10 v/v EQ Four Element Calibration Beads (Standard BioTools). .. Mass cytometry was performed using a Helios CyTOF instrument (Standard BioTools) operated with software v7.0.8493. ..

    Article Title: Investigating immune profile by CyTOF in patients with eosinophilic esophagitis after treatment with orodispersible budesonide.
    Article Snippet: Eosinophilic esophagitis (EoE) is a chronic Th2-mediated inflammatory disease of the esophagus driven by dietary or inhalant allergens which if left untreated, leads to fibrosis and poor esophageal function.. Although the inflammation in the esophagus is dominated by eosinophils, there are also elevated levels of T and B cells.. Blood samples from ten patients with EoE before and after treatment with orodispersible budesonide and 10 healthy controls were compared using cytometry by time-of-flight.

    Article Title: In vivo single-cell high-dimensional mass cytometry analysis to track the interactions between Klebsiella pneumoniae and myeloid cells
    Article Snippet: Cells were resuspended in CAS with 1 mM EDTA to a final concentration of 1 × 10 6 cells/mL, flushed through a 35 μM strainer, and supplemented with 1/10 v/v EQ Four Element Calibration Beads (Standard BioTools). .. Mass cytometry was performed using a Helios CyTOF instrument (Standard BioTools) operated with software v7.0.8493. ..

    Mass Cytometry:

    Article Title: In vivo single-cell high-dimensional mass cytometry analysis to track the interactions between Klebsiella pneumoniae and myeloid cells.
    Article Snippet: Cells were resuspended in CAS with 1 mM EDTA to a final concentration of 1 × 106 cells/mL, flushed through a 35 μM strainer, and supplemented with 1/10 v/v EQ Four Element Calibration Beads (Standard BioTools). .. Mass cytometry was performed using a Helios CyTOF instrument (Standard BioTools) operated with software v7.0.8493. ..

    Article Title: In vivo single-cell high-dimensional mass cytometry analysis to track the interactions between Klebsiella pneumoniae and myeloid cells
    Article Snippet: Cells were resuspended in CAS with 1 mM EDTA to a final concentration of 1 × 10 6 cells/mL, flushed through a 35 μM strainer, and supplemented with 1/10 v/v EQ Four Element Calibration Beads (Standard BioTools). .. Mass cytometry was performed using a Helios CyTOF instrument (Standard BioTools) operated with software v7.0.8493. ..

    Staining:

    Article Title: Targeting PD-L1 in cholangiocarcinoma using nanovesicle-based immunotherapy.
    Article Snippet: .. The cells were fixed with 2.6% PFA and stained with 125 nM cell-ID intercalator overnight at 4 C. The cells were washed twice and the data was acquired using a Helios CyTOF instrument (Standard BioTools, San Francisco CA). .. Data cleanup was performed using the Gaussian discrimination parameters in FlowJo and the samples were de-barcoded.

    other:

    Article Title:
    Article Snippet: FCS files were concatenated, normalized and debarcoded using Helios software (Fluidigm), and gated using FlowJo v.10 (BD Biosciences).



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    Image Search Results


    USP15 induces high MDSC infiltration and low CD8 + T-cell infiltration in the colorectal cancer microenvironment. A, Schematic illustration of CyTOF data acquisition. It is divided into five steps: tumor tissue collection, single-cell dissociation, cell staining, mass cytometry, and cell clustering and dimensionality reduction. B, Heatmap of the median marker intensities of the 41 markers in the nine cell populations. C, Nine cell populations obtained by manual merging in a t-SNE plot. D, t-SNE plot of cell infiltration in the USP15 WT ( n = 3) and Sg Usp15 ( n = 3) groups. E, Histogram of the nine cell populations in CD45 + cells in the WT and Sg Usp15 groups, respectively. F and G, The composition of MDSCs and CD8 + T cells in CT26 WT or CT26 Sg Usp15 tumors was evaluated by flow cytometry. H, Detection of infiltration by CD8α-positive cells and CD11b-positive cells in subcutaneous tumors was observed ( n = 5 per group, scale bar: 100 μm). Data are expressed as the mean ± SEM. Three independent replicates were performed. P values determined by ANOVA ( E ) or unpaired Student t test ( F , G , and H ). *, P < 0.05; ****, P < 0.0001; ns, not significant. DC, dendritic cell; DNT, double-negative T cell; gdT, gamma delta T cell.

    Journal: Cancer Immunology Research

    Article Title: USP15 Facilitates Colorectal Cancer Immune Evasion through SMYD3/CCL2-Dependent Myeloid-Derived Suppressor Cell Recruitment

    doi: 10.1158/2326-6066.CIR-24-1194

    Figure Lengend Snippet: USP15 induces high MDSC infiltration and low CD8 + T-cell infiltration in the colorectal cancer microenvironment. A, Schematic illustration of CyTOF data acquisition. It is divided into five steps: tumor tissue collection, single-cell dissociation, cell staining, mass cytometry, and cell clustering and dimensionality reduction. B, Heatmap of the median marker intensities of the 41 markers in the nine cell populations. C, Nine cell populations obtained by manual merging in a t-SNE plot. D, t-SNE plot of cell infiltration in the USP15 WT ( n = 3) and Sg Usp15 ( n = 3) groups. E, Histogram of the nine cell populations in CD45 + cells in the WT and Sg Usp15 groups, respectively. F and G, The composition of MDSCs and CD8 + T cells in CT26 WT or CT26 Sg Usp15 tumors was evaluated by flow cytometry. H, Detection of infiltration by CD8α-positive cells and CD11b-positive cells in subcutaneous tumors was observed ( n = 5 per group, scale bar: 100 μm). Data are expressed as the mean ± SEM. Three independent replicates were performed. P values determined by ANOVA ( E ) or unpaired Student t test ( F , G , and H ). *, P < 0.05; ****, P < 0.0001; ns, not significant. DC, dendritic cell; DNT, double-negative T cell; gdT, gamma delta T cell.

    Article Snippet: Following this, the cells were washed twice with Maxpar Water (Fluidigm, #201069), mixed with EQ Beads (Fluidigm, #201078), and analyzed using a cytometry by time-of-flight (CyTOF) instrument (Fluidigm, #Helios).

    Techniques: Staining, Mass Cytometry, Marker, Flow Cytometry